The Use of the Green Fluorescent Protein and Its Application in the Study of Biochemistry

Green Fluorescent ProteinApplications in Biochemistry, stall Biology and BiotechnologyThe fountain at large(p) protein (GFP) of Aequorea victoria is a alone(p) in vivo receptor for carry off dynamic processes in cells or organisms (Gerdes & Kaether 1996, p.44). This Northern Atlantic organism is highly organized in social system, with an amazing striking power to componentrate and emit green fluorescent light from photocytes at the margin of its umbrella, making it a perfect model for cellular studies. The molecular cloning of GFP and its evidence by Chalfie (1988) has opened up exciting new avenues of probe in cell biology. The massive flexibility as a non-invasive marker in mobile cells allows for numerous applications of GFP (Yang F. et al. 1996, p.1246) such as a reporter for constituent expression, as well as a marker to smasher cell lineage during development. Thus, its superpower to function as a protein tag allows us to concentre proteins in living cells, as it tolerates N- or C- terminal fusions to a broad variety of proteins (Tsien R.S. 1998, p.511). These applications of GFP ar now found to be more superior to alter methods in cell biology. GFP is a 238 amino acid protein with an naked molecular weight of most 27-30 kDa on SDS-PAGE (Gerdes & Kaether 1996, p.44).

The unique cylindrical fold of the protein seems ideally suited for its function, where two promoters baby buster together to form a dimer in the crystal, making it naming to undergo energy alter (Yang F. et al. 1996, p.1248). Eleven anti-parallel beta strands enjoyment up up the densely compact beta-barrel, where small sections of the of import whorl form interior caps on the cylinders (Prasher D.C. et al. 1992, p. 229). This irregular alpha spiraling segment provides a scaffold for the chromophore which is located in the nonrepresentational centre of the cylinder. The structure is... If you want to get a luxuriant essay, order it on our website: Ordercustompaper.com

If you want to get a full essay, wisit our page: write my paper